多粘芽孢杆菌发酵放大工艺研究
龙源期刊网 http://www.qikan.com.cn
多粘芽孢杆菌发酵放大工艺研究
作者:周荣华 崔怡宁 饶犇 廖先清 杨自文
来源:《湖北农业科学》2015年第11期
摘要:研究多粘芽孢杆菌的发酵放大工艺,探索在5t发酵罐中采用不同溶氧、不同碳源A浓度及不同种类的碳源对发酵的影响。结果表明,混用碳源B和碳源C可以较好的阻止多粘芽孢杆菌自溶,大部分菌体形成芽孢,发酵液中的芽孢数量为5亿/mL,这些研究为后面的工业化生产打下了良好的基础。
关键词:多粘芽孢杆菌(Paenibacillus polymyxa);放大;自溶
中图分类号:Q813.1 文献标识码:A 文章编号:0439-8114(2015)11-2634-02 DOI:10.14088/j.cnki.issn0439-8114.2015.11.019
Scale-up Fermentation of Paenibacillus polymyxa
ZHOU Rong-hua1,CUI Yi-ning2,RAO Ben1,LIAO Xian-qing1,YANG Zi-wen1
(1.National Biopesticide Engineering Research Center, Hubei Academy of Agricultural, Wuhan 430064, China;
2.Wuhan High School, Wuhan 430061, China)
Abstract: We studied the scale-up fermentation of Paenibacillus polymyxa. Different dissolved oxygen, different concentration of carbon source A and different types of carbon sources on the impact of fermentation were studied at 5 tons fermenter. The results showed that the carbon B
combined with carbon C can well prevent autolysis of Paenibacillus polymyxa and most bacteria can form spores. The number of spores in the fermentation broth was 500 million/mL. All these lay a good foundation for the industrialization.
Key words: Paenibacillus polymyxa; scale-up; autolysis
多粘类芽孢杆菌(Paenibacillus polymyxa)是芽孢杆菌科(Bacillaceae)类芽孢杆菌属(Paenibacillus)的革兰氏阳性细菌。多粘类芽孢杆菌细胞呈杆状,大小为(0.5~2.5) μm×(1.2~10.0) μm,G+C含量为40%~50%;利用周生鞭毛运动,在膨大孢子囊中产生椭圆型芽孢;最适生长pH为7,最适温度为28 ℃~30 ℃;兼性厌氧,能分解葡萄糖和其他糖类产酸,有时产气,在营养琼脂上无可溶性色素,应用前景广阔[1]。