mrs培养基配方
MRS（寒天）培养基一般是用来培养乳酸菌的，使用发明者的名字来命名的。啊！懒得翻译了，直接给你贴来了。
Often abbreviated to MRS, this type of bacterial growth medium is so-mamed by its inventors: de Man, Rogosa and Sharp. Developed in 1960, this medium was designed to favour the luxuriant growth of lactobacilli for lab study. It contains sodium acetate, which suppresses the growth of many competing bacteria.
Typical Composition (g/l)
Peptone, 10
Meat extract, 8
Yeast extract, 4
Glucose, 20
Sodium acetate 3H2O, 5
Tween 80, 1
Dipotassium hydrogen phosphate, 2
Triammonium citrate, 2
Magnesium sulfate 7H2O, 0.2
Manganese sulfate 4H2O, 0.05
Agar, 10
Final pH 6.2 @ 25°C
The yeast and meat extracts and peptone provide sources of carbon, nitrogen and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids specifically required by lactobacilli. Tween 80 is a surfactant which assists in nutrient uptake by lactobacilli. Magnesium sulfate and manganese sulfate provide cations used in metabolism.
mrs培养基的组织成分：
组成成 重 量(g)
牛肉蛋白粉 10
鱼肉汁 10
酵母浸出汁粉 5
葡萄糖 20
醋酸钠 5
柠檬酸二铵 2
吐温80 0.1
硫酸镁 0.58
硫酸锰 0.28
蒸溜水 1 000ml
备注：用高压锅在121℃灭菌15min，调节pH6.2～6.4
LC培养基只能计数干酪乳杆菌，MRS—水杨素（或山梨醇）培养基可以计数嗜酸乳杆菌和干酪乳杆菌；而MRS培养基可以计数这3种益生菌，通过减法原则从嗜酸乳杆菌、干酪乳
杆菌和双歧杆菌混合物中单独计数。另外，MRS—NNLP培养基也可用于选择性计数双歧杆菌。