云南师范大学生物信息学第一次上机实验报告
实验步骤：打开浏览器及百度首页，搜索NCBI 主页。在NCBI 主页中的搜索框输入pdgf AND “Mus musculus”, 点击search 。并限定选择为Nucleotide 。网站弹出下图
在search field 下的choose 中选择title ，于是出现下图
点击send, 选择file 及fasta 格式，将选中的序列下载到本地并用记事本打开。
所下载的序列如下：
>gi|758768|gb|U20282.1|MMU20282 Mus musculus stromelysin PDGF responsive element binding protein transcription factor mRNA, complete cds
TAGAAA TGAGAAGTCCCCAAGTCTTCTACAGGAAGTGCTTCAGGGCTACCACCACCAT CCTGACAGAAGG
TA TCCTAGAAGCGCACAGGAACA TCAAGGGA TGGCTAGTGGCCTGGAAGGAACTGCA AGGCCTAACATCT
TAGTCAGTCAAACCAA TGAA TTAGCTAGCAGCGGCCTTCTGAACAAGACTA TTGGGCC CCTGTTGGAAAA
CCCCCACTGGACCACGGGAAAGGAAGTCAAGCAGCACAGCTCCTGAAATGAAACAGA TCAA TTTGTCTGA
CTATCCCATTCCCAGGAAGTTTGAGA TAGAACCTCCATCATCAGCCCATGAGCCTGGAG GCTCCCTTTCT
GAAAGGAGGTCAGTAA TCTGTGA TA TATCCCCACTAAGACAGATTGTCAGGGACCCAG GGGCTCACTCAT
TGGGACACATGGGTACTGA TGCCAGAATTGGGAGGAA TGAACGTCTCAACCCAAGTTT AAGTCAGTCAGT
CA TTCTTCCTGGTGGGTTGGTGTCCATGGAAACAAAA TTGAAA TCCCAGGGTGGACAG ATAAAAGAGGAA
GACTTTGAACAA TCCAAATCTCAAGCTAGTTTCAACAAGAAA TCTGGAGACCACTGCC ATCCTACCAGCA
TCAAACATGAGACTTACCGTGGCAATGCAATGCAAGTCCTGGAGCAGCAGCCCATGA T TCCA TTTCAGAC
TA TGGCCCACAAGA TAGCAGGTCCACACCAATGCGGCGGGTCCCTGGTATAGTTGGTA GCCGGGAGACTA
TGAGGGGTCGGTCCTCTTCTCAGTACCA TGACTTTGCAGAAAAA TTGAAGA TGTCTCC AGGCAGGAGCAG
AGGCCCAGGGGGAGACCCTCA TCACA TGAACCCACATA TGACCTTTTCAGAGAGGGCC AA TAGGAGTTCT
TTACA TGCTCCTTTTTCTCGGAACTCAGAAAGCCTGGCCTCTGCTTACCACACAAACAC CAGGGCTCATG
CTTATGGGGACCCTAATACCGGTTTGAA TTCCCAGCTCCATTA TAAGAGACAGA TGTAC CAACAGCAACA
AGAGGAGTATAAAGACTGGGCCAGCAGTTCTGCTCAGGGAGTGATCGCTGCTGGCGA T AGGCAGGAAGGC
CCACGCAAGAGCCCAAGGCAGCAGCAGTTTCTTGACAGAGTAGGGAGCCCCCTGAAA AA TTACAAAGGCG
GAATGA TGTATGGCCCACCAGTAGGTACGTATCA TGACCCAAGCACTCAGGAAGCTGG GCGCTGTCTCAT
GTCTAGTGATGGTCTGCCTGCCAAAAGCA TGGAATTGAAGCACAGCTCTCAGAAGTTA CAAGAGTCTTGT
TGGGATCTTTCTCGGCAGACTTCTCCAGCCAAAAGCAGTGGCCCTCCAGGAA TGTCTA ATCAAAAACGGT
ATGGGCCACCCCATGAGCCAGA TGGACA TGGACTAGCCGAGTCTGCACAGTCATCCAA ACCTAGTAACGT
CA TGTTACGGCTTCCTGGTCAAGAGGA TCA TTCTTCTCAAAATCCTTTAATCATGCGAA GGCGGGTTCGT
TCTTTTATCTCCCCTA TTCCCAGTAAGAGACGGTCACAAGATGTAAAAAACAGTAACGC TGATGATAAAG
GGCGCCTCCTCCACCCA TCAAAAGAAGGTGCCGATAAAGCATACAATTCCTACAGCCA TCTTTCTCACAG
TCAGGATATCAAGTCTA TTCCTAAGAGAGATTCCTCCAAGGACCTTCCAAACCCAGACA ATAGAAACTGT
CCTGCTGTTACCCTGACAAGTCCTGCTAAGACCAAAATACTGCCCCCACGGAAGGGAC GGGGATTAAAAT
TGGAAGCTATAGTTCAGAAGATCACA TCTCCAAA TA TTAGGAGGAGTGCATCTGCAAAC AGTGCTGAGGC
TGGGGGAGACACAGTCACACTGGATGA TA TACTATCTCTAAAGGGTGGTCCTCCTGAA GGTGGCACTGTG
GCTACTCAAGAGGCTGAGATGGAAAAGCGAAAGTGTGAGGTGGTA TCTGACCTAGTC AGTGTAACTAACC
AGGAATCGAATGTTGAGAAGCCTCTTCCAGGGCCTTCTGAAGAGTGGCGTGGCAGTG GGGATGACAAAGT
CAAGACAGAGGCACA TGTAGAAACAGCTTCTACTGGAAAGGAACCCTCTGGTACTATG ACATCCACAGCT
TCACAGAAGCCTGGTGGTAACCAAGGGAGACCAGA TGGTTCCCTGGGTGGGGCAGCA CCACTAATCTTTC
CTGACTCAAAGAA TGTAGCTCCAGTGGGCA TATTGGCCCCTGAGGCAAATCCCAAGGC TGAAGAGAAAGA
GAACGA TACAGTCATGA TTTCACCCAAACAAGAAAGTTTCCCCCCTAAAGGGTATTTCC CA TCAGGAAAG
AAAAAGGGGAGACCAA TCGGTAGTGTCAATAAGCAAAAGAAACAGCAGCAGCAACC ACCTCCACCTCCTC
AGCCCCCTCAGA TGCCAGAAGGTTCTGCAGATGGGGAGCCAAAGCCAAAAAAGCAGA GGCAAAGGAGGGA
GAGAAGGAAGCCTGGGGCCCAGCCAAGGAAGCGGAAAACCAAACAAGCAGTTCCCA TTGTAGAACCTCAA
GAACCAGAGATCAAGCTAAAGTACGCTACCCAGCCACTAGATAAAACTGA TGCCAAGA ACAAGTCTTTTT
TCCCTTACATCCATGTAGTAAATAAGTGTGAACTTGGAGCTGTTTGTACAATCATCAATG CTGAAGAAGA
AGAACAGACCAAATTGGTGAGGAGCCGGAAGGGTCAGAGATCTCTGACCCCTCCCCC CAGCAGCACAGAA
AGCAAGGTGCTCCCAGCTTCATCCTTTATGCTGCAGGGGCCTGTGGTAACAGAGTCTT CTGTTATGGGGC
ACCTGGTTTGCTGTCTCTGTGGCAAGTGGGCCAGTTACCGTAACA TGGGTGACCTCTTT GGACCCTTTTA
TCCCCAAGATTATGCAGCCACTCTTCCGAAGAATCCACCTCCTAAGAGGTCCTCAGAA ATGCAGAGCAAA
GTCAAGGTTCGGCACAAAAGCGCTTCTAATGGTTCTAAAACTGACACTGAGGAGGAG GAGGAGCAGCAGC
AGCAGAAGGAGCAGAGGAGCCTGGCTGCTCATCCTAGGTTCAAGCGGCGCCACCGCT CAGAAGACTGTGG
TGGAGGTCCTCGGTCCCTGTCCAGGGGGCTCCCTTGTAAAAAAGCAGCCACGGAGGG CAGCAGTGAAAAG
ACTGTTTCAGACACAAAGCCCTCTGTACCTACCACTTCAGAAGGTGGGCCTGAGCTGG AGTTACAAATCC
CTGAACTACCTCTTGACAGCAACGAATTTTGGGTCCATGAGGGTTGTATTCTCTGGGCC AA TGGAATCTA
CCTGGTCTGTGGCAGGCTCTATGGCCTGCAGGAAGCGCTGGAAATCGCCAGAGAGATG AAATGCTCCCAC
TGCCAGGAGGCTGGCGCTACTTTGGGCTGCTACAACAAGGCTGCTCCTTCCGATACCA TTACCCATGTGC
CA TTGACGCAGA TTGTTTGCTGCA TGAGGAGAA TTTCTCGGTGAGGTGCCCCAAGCAC AAGGTGAGACTG
TGGGGATGAGAAGGTGGAGGACACACGTGACGGAA TGGAATCCTCCTGCTGTGCAAC CACACCACTCTGC
CCTGCCCTGCCCTGCACCCCCGCTGCCGTGCGCCTGCCCATGCCAGCACTTCCTTCCTA TGTTCTCACAT
CACACTCAAACTGGTGACACCACAGGAAAAGAAAGATACAAGATGTTGGAATGGCTG TTTCCA TGGACAC
AA TCTCCATAGTGACAA TGTGGGGGAGGGGGGAGGGGAGGGCTGATGGGGAAACGGG GAGTTAAAAGGGA
GGGACAAAAAATAATA TATATATAAA TCTATTTTTAGTCTGGAAAGACTTTGTTTAAA TG AAAGGTGCGC
TA TCCCTTTTGATTCTATTTTAAAATTATCTAGTTAATTCAAAA TTTGTTCCAATGACAAT GAAATTTAA
ATGTG
即