氧化苦参碱对环磷酰胺增效减毒作用

中华中医药杂志(原中国医药学报) 2012年7月第27卷第7期 CJTCMP , July 2012,Vol . 27, No. 7• 1901 •

·研究报告·

氧化苦参碱对环磷酰胺增效减毒作用

梁冰，李淑芳，王骁宇，刘锦红，沈祥春

（贵阳医学院，贵阳 550004）

摘要：目的：探讨氧化苦参碱对环磷酰胺（CTX）抗S180小鼠的增效减毒作用。方法：建立S180荷瘤小鼠模型，增效实验设立：模型组、环磷酰胺10mg/kg组、环磷酰胺20mg/kg组、环磷酰胺（5mg/kg）+氧化苦参碱（50mg/kg）组、环磷酰胺（10mg/kg）+氧化苦参碱（50mg/kg）组，观察瘤重、抑瘤率、胸腺指数和脾脏指数。减毒实验设立：正常对照组、环磷酰胺100mg/kg组、环磷酰胺（100mg/kg）+氧化苦参碱低剂量（25mg/kg）组、环磷酰胺（100mg/kg）+氧化苦参碱中剂量（50mg/kg）组、环磷酰胺（100mg/kg）+氧化苦参碱高剂量（100mg/kg）组，观察白细胞数、胸腺指数和脾脏指数，检测肝、肾功能。结果：增效实验中，各用药组与模型组相比肿瘤抑制率明显提高（P

关键词：氧化苦参碱；环磷酰胺；增效；减毒

基金资助：国家自然科学基金项目（No.30701024，No.81173586），贵州省科技攻关项目（No.黔科合SY字

[2011]3010号），贵州省国际科技合作计划项目（No.黔科合外G字[2009]700115号）

Synergism and attenuation effects of oxymatrine on cyclophosphamide

LIANG Bing, LI Shu-fang, WANG Xiao-yu, LIU Jin-hong, SHEN Xiang-chun

(Guiyang Medical University, Guiyang 550004, China)

Abstract: Objective: To investigate synergism and attenuation effects of oxymatrine (OMT) on cyclophosphamide (CTX) anti-S180 tumor-bearing mice. Methods: The model of S180 tumor-bearing mice was established. The groups of synergism experiment were divided into 5 as following: the model group, cyclophosphamide 10mg/kg, 20mg/kg group, cyclophosphamide (5mg/kg) +OMT (50mg/kg) group, cyclophosphamide (10mg/kg) +OMT (50mg/kg) group. The following indexes were detected including: the tumor weight,tumor inhibition rate, thymus index, and spleen index. There were 6 groups designing in attenuation experiment as following: control group, cyclophosphamide 100mg/kg group, cyclophosphamide (100mg/kg) + oxymatrine low-dose (25mg/kg) group, cyclophosphamide (100mg/kg) + oxidation middle-dose (50mg/kg) group, cyclophosphamide (100mg/kg) + oxymatrine high-dose (100mg/kg) group. White blood cell count, thymus index and spleen index were be assayed. Liver and kidney function were detected by marketable kits. Results: In the synergism experiments, the treatment group could significantly improve tumor inhibition rate compared with the model group, the index thymus in binding groups were increased compared with cyclophosphamide. In attenuation experiments: the number of white blood cells was increased in oxymatrine high-dose and low-dose combination with cyclophosphamide group which is significantly difference compared with cyclophosphamide group. The activities of aspartate and alanine aminotransferase were significantly decrease in Cyclophosphamide combination with Oxymatrine compared with Cyclophosphamide treatment only. Conclusion: Combined with cyclophosphamide, Oxymatrine not only can improve the inhibition rate and white blood cell count, but also reduce the cyclophosphamide-induced liver damage.

Key words: Oxymatrine; Cyclophosphamide; Synergism; Attenuation

Fund assistance: National Natural Science Foundation of China(No. 30701024, No.81173586), Provincial Key Technologies R&D Program of Guizhou (No. [2011]3010), International Science and Technology Exchange and Cooperation of Guizhou Province (No.2009-700115)

通讯作者：沈祥春，贵州省贵阳市北京路9号贵阳医学院药理学教研室，邮编：550004，电话：0851-6908108E -mail：[email protected]