巨大芽孢杆菌发酵培养基的优化
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巨大芽孢杆菌发酵培养基的优化
作者：饶犇 王亚平 李娜 周荣华 廖先清 刘芳 陈伟 张光阳 杨自文
来源：《湖北农业科学》2014年第15期
摘要：为了得到巨大芽孢杆菌（Bacillus megaterium）发酵的最佳培养基，在单因子试验的基础上，应用Plackett-Burman设计法对影响芽孢产量的基本培养基组分中的关键因子进行了筛选，并进一步采用响应面分析法（RSM）对影响芽孢产量的关键因素最佳水平作了深入的研究。结果表明，影响巨大芽孢杆菌芽孢产量的关键因素是豆粕和淀粉的浓度。通过RSM的拟合和推算得到在豆粕和淀粉浓度分别为48.85和19.67 g/L时，此时模型预测发酵最佳产量为43.16×108 CFU/mL，验证值为42.2×108 CFU/mL，预测值与验证值之间吻合较好，比原始培养基提高了约10倍。
关键词：巨大芽孢杆菌（Bacillus megaterium）；Plackett-Burman设计法；响应面分析法（RSM）
中图分类号：Q813.1 文献标识码：A 文章编号：0439-8114（2014）15-3539-03 Optimizing Fermentation Conditions of Bacillus megaterium
RAO Ben1，WANG Ya-ping2， LI Na3， ZHOU Rong-hua1，LIAO Xian-qing1，LIU Fang1，CHEN Wei1，ZHANG Guang-yang1，YANG Zi-wen1
（1.Biological Pesticide Engineering Research Center，Hubei Academy of Agricultural
Sciences， Wuhan 430064，China；2.Key Lab of Industrial Biotechnology， Hubei University， Wuhan 430062，China；3. Department of Life Science and technology/Yunan Provincial Key lab of Crop Quality and Efficient Cultivation and Safety Control， Red River Insitute，Mengzi 661100，Yunan，China ）
Abstract： To obtain the optimal medium for producing Bacillus megaterium spore， statistical method was used to investigate the medium components for spore concentration and productivity by Bacillus megaterium. The Plackett-Burman design was used to evaluate the effects of six variables. The results showed that the optimal values of the two parameters determined by RSM were soya bean meal of 48.85 g/L and starch of 19.67 g/L. The spore production was predicted to be 43.16×108
CFU/mL， and the actual value was 42.2×108 CFU/mL. They were fitted well with 10 folds increase. Key words： Bacillus megaterium； Plackett-Burman； response surface analysis methodology （RSM）
收稿日期：2013-11-05