pYES2酵母表达载体说明

pYES2

编号

北京华越洋生物VECT2980

载体基本信息

出品公司:

载体名称:

质粒类型:

表达水平:

诱导方法:

启动子:

克隆方法:

载体大小:

5'

测序引物及序列:

3'

测序引物及序列:

载体标签:

载体抗性:

筛选标记:

备注:

产品目录号:

稳定性:

组成型/诱导型:

病毒/非病毒:

载体质粒图谱和多克隆位点信息

载体名称

pYES2

Invitrogen

pYES2

酿酒酵母蛋白表达载体

高拷贝

半乳糖

GAL1

多克隆位点，限制性内切酶

5857

b p

T7:

T AATACGACTCACTATAGGG

CYC1

T erminator:

G TGACATAACTAATTACATGATG

/

氨苄

URA3

利用半乳糖诱导蛋白在酿酒酵母中表达。

-­‐-­‐

稳定

Stable

诱导型

非病毒

pYES2多克隆位点

载体简介

pYES2的是一个5.9

kb的载体，设计用来在酿酒酵母（Saccharomyces

cerevisiae）中诱导表达重组蛋白。载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进行转化株的筛选。

该载体包含以下元素：

1.

酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达

2. 多克隆位点可以使用的很多限制酶切位点，便于基因插入。

3.CYC1终止子能够有效终止mRNA 的转录。

4. 能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。

5. 氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。

载体序列

LOCUS pYES2 5857 bp

DEFINITION pYES2

ACCESSION

KEYWORDS

SOURCE

ORGANISM other sequences; artificial sequences; vectors.

COMMENT This file is created by Vector NTI

FEATURES Location/Qualifiers

source 1..5857

/organism="pYES2"

/mol_type="other DNA"

terminator complement(12..251)

/label="CYC1_terminator"

misc_feature 218..236

/label="pYESTrp_rev_primer"

misc_feature 218..236

/label="CYC1_primer"

promoter complement(368..386)

/label="T7_promoter"

promoter complement(410..861)

/label="GAL1_promoter"

misc_feature complement(424..447)

/label="GAL1_primer"

rep_origin complement(1402..2873)

/label="2micron_origin"

rep_origin 1404..2283

/label="2micron2_origin"

promoter 2876..3101

/label="URA3_promoter"

gene 3103..3903

/label="URA3"

/gene="URA3"

CDS 3103..3906

/label="ORF frame 1"

CDS complement(3138..3614)

/label="ORF frame 3" DNA circular SYN

gene 4002..4862

/label="Ampicillin (variant)"

/gene="Ampicillin (variant)"

CDS 4002..4625

/label="ORF frame 3"

rep_origin 5017..5636

/label="pBR322_origin"

ORIGIN

1 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA 61 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG 121 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA 181 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA 241 CTAATTACAT GATGCGGCCC TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT 301 CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT CCGAGCTCGG TACCAAGCTT 361 AATATTCCCT ATAGTGAGTC GTATTACAGC TGCTAGTAGT CCGATCCGGG GTTTTTTCTC 421 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG 481 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TAATGCAGTT 541 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA 601 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA 661 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA 721 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA 781 ACGCGACCGG TGAGGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC 841 GCTCGGCGGC TTCTAATCCG TACTAGTGGA TCATCCCCAC GCGCCCTGTA GCGCCCCATT 901 AAGCGCGGCG GGTGTGGTGG TTACGCCCAG CGTGACCCCT ACACTTCCCA CCGCCCTAGC 961 CCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 1021 AGCTCTAAAT GGGGGCATCC GTTTACCCTT CCGATTTACT GCTTTACGGC ACCTCGACCC 1081 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACCCTTTT 1141 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTGG AAACTGGAAC 1201 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGG 1261 CTATTCGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 1321 AACGTTTACA ATTTAAATAT TTGCTTATAC AATCTTCCTG TTTTTGGGGC TTTTCTGATT 1381 ATCAACCGGG GTGGAGCTTC CCATTGCGAA TACCGCTTCC ACAAACATTG CTCAAAAGTA 1441 TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC CTACCCATCC ACCTTTCGCT 1501 CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT GTTTATCTCT AGTATTACTC 1561 TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG AATCGAAAAC AATACGAAAA 1621 TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG AAGAAACCGT TCATAATTTT 1681 CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC ACAAAGTATG CGCAATCCAC 1741 ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA AAATGCACCC GCAGCTTCGC 1801 TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT TATGGAAGAG AAAATAGACA 1861 CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC AAAAAGTTAT CAAGAGACTG 1921 CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA TGCTTTGTTA GAAAAATAGC 1981 GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA GATTCTTTGT TGGTAAAATA 2041 GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA 2101 TTAGCGCTCT CGTCGCGTTG CATTTTTGTT TTACAAAAAT GAAGCACAGA TTCTTCGTTG

2161 GTAAAATAGC GCTTTCGCGT TGCATTTCTG TTCTGTAAAA ATGCAGCTCA GATTCTTTGT 2221 TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTCTACA AAATGAAGCA CAGATGCTTC 2281 GTTAACAAAG ATATGCTATT GAAGTGCAAG ATGGAAACGC AGAAAATGAA CCGGGGATGC 2341 GACGTGCAAG ATTACCTATG CAATAGATGC AATAGTTTCT CCAGGAACCG AAATACATAC 2401 ATTGTCTTCC GTAAAGCGCT AGACTATATA TTATTATACA GGTTCAAATA TACTATCTGT 2461 TTCAGGGAAA ACTCCCAGGT TCGGATGTTC AAAATTCAAT GATGGGTAAC AAGTACGATC 2521 GTAAATCTGT AAAACAGTTT GTCGGATATT AGGCTGTATC TCCTCAAAGC GTATTCGAAT 2581 ATCATTGAGA AGCTGCAGCG TCACATCGGA TAATAATGAT GGCAGCCATT GTAGAAGTGC 2641 CTTTTGCATT TCTAGTCTCT TTCTCGGTCT AGCTAGTTTT ACTACATCGC GAAGATAGAA 2701 TCTTAGATCA CACTGCCTTT GCTGAGCTGG ATCAATAGAG TAACAAAAGA GTGGTAAGGC 2761 CTCGTTAAAG GACAAGGACC TGAGCGGAAG TGTATCGTAC AGTAGACGGA GTATCTAGTA 2821 TAGTCTATAG TCCGTGGAAT TAATTCTCAT CTTTGACAGC TTATCATCGA TAAGCTAGCT 2881 TTTCAATTCA ATTCATCATT TTTTTTTTAT TCTTTTTTTT GATTTCGGTT TCTTTGAAAT 2941 TTTTTTGATT CGGTAATCTC CGAACAGAAG GAAGAACGAA GGAAGGAGCA CAGACTTAGA 3001 TTGGTATATA TACGCATATG TAGTGTTGAA GAAACATGAA ATTGCCCAGT ATTCTTAACC 3061 CAACTGCACA GAACAAAAAC CTGCAGGAAA CGAAGATAAA TCATGTCGAA AGCTACATAT 3121 AAGGAACGTG CTGCTACTCA TCCTAGTCCT GTTGCTGCCA AGCTATTTAA TATCATGCAC 3181 GAAAAGCAAA CAAACTTGTG TGCTTCATTG GATGTTCGTA CCACCAAGGA ATTACTGGAG 3241 TTAGTTGAAG CATTAGGTCC CAAAATTTGT TTACTAAAAA CACATGTGGA TATCTTGACT 3301 GATTTTTCCA TGGAGGGCAC AGTTAAGCCG CTAAAGGCAT TATCCGCCAA GTACAATTTT 3361 TTACTCTTCG AAGACAGAAA ATTTGCTGAC ATTGGTAATA CAGTCAAATT GCAGTACTCT 3421 GCGGGTGTAT ACAGAATAGC AGAATGGGCA GACATTACGA ATGCACACGG TGTGGTGGGC 3481 CCAGGTATTG TTAGCGGTTT GAAGCAGGCG GCAGAAGAAG TAACAAAGGA ACCTAGAGGC 3541 CTTTTGATGT TAGCAGAATT GTCATGCAAG GGCTCCCTAT CTACTGGAGA ATATACTAAG 3601 GGTACTGTTG ACATTGCGAA GAGCGACAAA GATTTTGTTA TCGGCTTTAT TGCTCAAAGA 3661 GACATGGGTG GAAGAGATGA AGGTTACGAT TGGTTGATTA TGACACCCGG TGTGGGTTTA 3721 GATGACAAGG GAGACGCATT GGGTCAACAG TATAGAACCG TGGATGATGT GGTCTCTACA 3781 GGATCTGACA TTATTATTGT TGGAAGAGGA CTATTTGCAA AGGGAAGGGA TGCTAAGGTA 3841 GAGGGTGAAC GTTACAGAAA AGCAGGCTGG GAAGCATATT TGAGAAGATG CGGCCAGCAA 3901 AACTAAAAAA CTGTATTATA AGTAAATGCA TGTATACTAA ACTCACAAAT TAGAGCTTCA 3961 ATTTAATTAT ATCAGTTATT ACCCATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 4021 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 4081 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 4141 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 4201 TGAGCACTTT TAAAGTTCTG CTATGTGATA CACTATTATC CCGTATTGAC GCCGGGCAAG 4261 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 4321 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 4381 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 4441 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 4501 TGAATGAAGC CATACCAAAC GACGAGAGTG ACACCACGAT GCCTGTAGCA ATGCCAACAA 4561 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 4621 ACTGAATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 4681 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 4741 TGGGGCCAGA TGGTAAGCGC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA

4801 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 4861 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 4921 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 4981 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 5041 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 5101 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 5161 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 5221 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 5281 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 5341 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 5401 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 5461 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 5521 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 5581 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 5641 TTTTACGGTT CCTGGGCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 5701 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 5761 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 5821 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAG

//

其他酵母表达载体：

p416GFD

p53blue

pACT2-AD

pAD-GAL4-2.1

pADH2

pAUR123

pBridge

pCL1

pDEST32

pDisplay

pDR195

pESC-His

pESC-Leu

pESC-TRP

pESC-URA

pFLD

pFLD/CAT

pFLD α

pGADT7-T

pGAG424

pGAPZA

pGAPZB pPIC9 pPIC9K pPIC9k-His pPICZA pPICZB pPICZC pPICZ αA pPICZ αB pPICZ αC pPICZ αD pPICZ αFC pPICZ αGB pPink-HC pPink-LC pPink α-HC pRS403 pRS405 pRS406 pRS414 pRS415 pRS416 pRS41H pFA6a-FGP(S65T)-kanMX6 pRS316

pGAPZ αB

pGAPZ αC

pGBKT7

pGBKT7-53

pGBKT7-Lam

pHIC-PI

pHIL-D2

pHIL-S1

pHis2

pHisSi-1

pMETA

pMETB

pMETC

pMET αA

pMET αB

pMET αC

pMyr

pPIC3.5

pPIC3.5K

pPIC6B

pPIC6C

pPIC6αA

pPIC6αB

pPIC6αC

pYX212

SUMOprotease

Ycp22lac-EGFP pRS426 pRS426gal pSEP1 pSEP2 pSEP3 pSos pSos-MAFB pUG66 pYC2/CT pYC2/NTA pYC2/NTB pYCP211 pYEPlac112 pYEPlac195 pYES2 pYES2-EGFP pYES2-kan pYES2-NTA pYES2-NTB pYES2-NTC pYES3/CT pYES6/CT pYES-DEST52pYIP211 pYIP5 pYRP7 Ycplac33