第三代测序技术:单分子即时测序
第三代测序技术:单分子即时测序
刘岩
吴秉铨
DNA测序技术是分子生物学研究中最常用的技术,它的出现极大地推动了生物学的发展。从人类基因组计划(human genome project),到人类基因组单倍型图计划(HapMap),再到人类癌症基因组及个体基因组计划,第一代和第二代DNA测序技术功不可没。特别是近几年发展起来的第二代DNA测序技术则使得DNA测序进入了高通量、低成本的时代。目前,基于单分子读取技术的第三代测序技术已经出现,该技术测定DNA序列更快,并有望进一步降低测序成本,为人类从基因水平深入理解疾病的发生、发展、诊断和治疗提供新的手段,使个体化医疗成为现实。本文回顾了测序技术发展过程,并对第三代测序技术的特点和优势进行详细论述。
10.3760/cma. j. issn. 0529-5807. 2011.10.022
100191 北京大学医学部病理学系
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2011-03-14
第三代测序技术:单分子即时测序
作者:作者单位:刊名:英文刊名:年,卷(期):
刘岩, 吴秉铨
100191,北京大学医学部病理学系中华病理学杂志
Chinese Journal of Pathology2011,40(10)
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